01 January 1970 1 9K Report

fibrinogen definition with biological role

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Can I use the result from a pilot study to decide the minimum sample size ?

Can I use the result from a pilot study to decide the minimum sample size required to compare the means using independent sample t-test ? Or any other way that can help me to determine the...

29 January 2021 7,677 6 View

Yield of Plasmid is low in Maxipreparation of plasmid for 200ml? What might be the reasons for it?

Hi, I am working with a plasmid which is known to have very low copy number and when I am isolating it by midiprep alkaline lysis method, I am getting good amount of plasmid i.e., 16.56ng/ul....

11 December 2020 1,369 3 View

How to fix the fungus for SEM imaging?

I want to perform the SEM of candida sp. and i want to know that the fixation for the bacteria and fungus are different or same?

10 December 2020 6,169 3 View

Suggest a reasonable GPU for Mbile Workstation (Laptop)?

I am looking for a reasonable GPU for a Mobile Workstation (Laptop) which can run most State-of-the-art neural networks for images (Computer Vision). Specifically, if anyone has some information...

24 November 2020 2,878 2 View

Meshing Problem in Comsol.?

I am working on electrical streamer discharge but it gives errors. Anybody can help me out.Kindly

18 October 2020 5,672 2 View

How we could use Machine Learning/ Deep Learning in channel estimation?

I am new to ML/DL domain and my area of research is channel estimation in Massive MIMO. I would like to know a few things: 1) Can we use ML/DL algorithms to get optimal channel estimates in...

14 August 2020 9,803 3 View

Why eluted protein shows degradation though beads after elution is still clean ?

I tried to purified a GST-tagged protein and I used 0.5% triton-x-100 to wash the enriched beads which resulted in a removal of non specific binding to a great extent. I eluted the protein with...

04 August 2020 3,701 6 View

What is the minimum sample size that is acceptable to conduct a survey?

I'm doing a study to evaluate the adherence rate of clinicians to a clinical practice guideline using clinical vignettes (an online survey). The study only includes physicians who are dealing with...

31 July 2020 5,804 4 View

Will genetic characteristics of bacterial isolate have stored for long time can change such as plasmid loss?

I hope get answers with evidence

02 May 2020 707 46 View

How to apply the non-reflection boundary for Lamb wave propagation in anisotropic laminates in order to delete the reflections from plate edge?

Hi guys, I hope you are in the best of your health. I am working on Lamb wave propagation in anisotropic composite laminates. I want to apply the non-reflection boundary conditions in the...

25 March 2020 4,358 5 View

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We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

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What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

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Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

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Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Can MicroRNA cross the blood brain barrier?

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