What's the most processive PCR enzyme?

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Is there a solution to include right and left censoring in one survial analysis ?

Dear all ! I'm working on historical data related to events and like to carry out a survival analysis. In the dataset there are typically some right censored data, when the individuals are still...

15 May 2023 2,841 2 View

How to fix both serial and cross-section auto correlation in panel date at the same time?

Hi! How do I fix both serial and cross-section auto correlation in panel date at the same time? I run a fixed effects model with PSCE errors (takes care of cross-sectional autocorrelation), and...

12 November 2022 3,190 1 View

DOSY - reliable processing?

While performing DOSY, I noticed something strange that I can't explain: I performed 2 DOSYs of 2 polymers with exactly the same parameters (pulse sequence, small and big delta, temperature, same...

30 August 2022 230 1 View

What statistical method to use to use when you have 3 groups?

I'm in the planing phase of doing a trail for a new therapy treatment for generalized anxiety with 3 groups. A treatment as usual as usual group, and a wait-list control, both serve as a control...

26 June 2022 8,237 5 View

How can i find the risk free rate weekly?

Dear all, I'm working on a Markowitz portfolio on carbon credits. These have not been around for the recommended 30 years. so I changed the data points from monthly to weekly to at least have a...

10 February 2022 9,009 0 View

When to work with aggregated data for a linear regression?

I am trying to do a linear regression with walking speed of pedestrians as the dependent and population size as the independent variable. I have collected the same amount of samples (200) for each...

13 January 2022 3,602 11 View

28 observations for a regression?

I only have 28 observations for a regression, while the minimum is often said to be 30. Is that ok anyway, and are there any extra security measures I can take, such as more stern application of...

07 January 2022 4,359 5 View

How does exogenous variables enter in a panel ARDL model?

Like the title says - where do I put exogenous variables in a panel ARDL model - in the long-run or short-run equation? I want to control for world war one, which means either impulse dummies for...

04 January 2022 348 1 View

Right approach for cointegration when mixing stationary and non-stationary variables in panels?

Hi! I want to mix stationary and non stationary panel data in an error correction and for cointegration. In a non-panel data setting, this is done with ARDL ECM, where a significant EC and...

20 May 2021 1,487 3 View

Why is there no constant in the long-run in panel ARDL (MPG) regression?

Why is there no constant in the long-run in panel ARDL (MPG) regression? It is the same in Eviews and Stata.

20 May 2021 9,371 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Identify the eight key processes of excellent supply chain management and discuss how each of these processes impacts the end customer?

Read the journal article by Douglas M. Lambert, “The Eight Essential Supply Chain Management Processes,” Supply Chain Management Review, Vol. 8, No. 6 (2004), pp. 18-26

04 August 2024 9,919 4 View

Does atmosphere affect flow of matter & energy on Earth & flow of energy in biosphere related to the flow of food through a food chain?

How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?

02 August 2024 9,644 0 View

Could someone explain the basics of working with enzymes?

I'm interested in learning about enzyme usage. Could someone explain the basics of working with enzymes? I understand they're different from standard chemicals, but I'm uncertain about the...

01 August 2024 5,460 5 View