What's the maximum percentage of DMSO allowed for SPR running buffer?

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Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

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Arnoud Marquart

In principle the instrument (IFC of Biacore) can withstand short pulses of 100% DMSO, although not recommended. I would keep the DMSO concentration in the flow buffer as low as possible. Say below 5% and replace the flow buffer as soon as possible with buffer without DMSO when done.

Thomanai Lamtha

Thank you sir,

(My instrument is from Nicoya Lifesciences)