Thomanai Lamtha

3 Questions 9 Answers 0 Followers

Questions related from Thomanai Lamtha

How generally does the IC50 from a cell-based assay different(higher) from an enzymatic assay?

I always got IC50 of small molecule TKIs from cancer cells viability assay(MTT, Alama blue) 50 – 200 folds higher than IC50 from enzyme inhibition assay. What factors affect them?

26 May 2021 8,045 3 View

What's the maximum percentage of DMSO allowed for SPR running buffer?

My analyte is small molecule drugs

13 February 2019 354 2 View

Should 8 M urea buffer be autoclaved for inclusion bodies solubilization?

I have ever autoclaved an extraction buffer with 8 M urea for solubilize my protein that expressed in IB form, it so smelly (urea breaks down when heated, releasing NH3) and I worry about protein...

22 August 2017 5,256 8 View