Hello dear members,
I want to build homology model for my target protein while using online server where the algorithm should be based on threading, because the available templates from PDB for my target protein has < 40 % seq. identity. I came to know about two servers i-e. I-TASSER and LOMETS , but I'm pretty confuse that what's the difference between these two servers and which one might be preferred option to use for homology modeling.
Thanks in advance!
Hello!
"The I-TASSER server in general has models with a higher accuracy than LOMETS, since I-TASSER has extensive structure refinement on the templates. This is the reason that I-TASSER takes a longer time to generate the refined models. But the difference is small if there is a close homology identified by LOMETS, which is indicated by the column of "Confidence Score" ("High" usually indicates a close homology). The difference becomes larger if LOMETS cannot identify a close homologous template, where I-TASSER manages to build some part of proteins by ab initio folding." http://zhanglab.ccmb.med.umich.edu/LOMETS/FAQ.html
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Biomolecules
- Proteins