Is someone working with the CFX96Touch Real-TimePCR System (Biorad)?
How is your experience with this system? It is our first option for multiplexing qPCR systems because it is in our budget range price.
Other options are:
CFX96Touch Real-TimePCR (Biorad)
QuantStudio 3 (Thermo)
StepOnePlus (Thermo)
We are interested in at least 5 channels, robust performance, versatility, cost of reagents, and excellent customer support. What to think about them?
Thanks a lot in advance!
I have used that qPCR machine and it works very well. I used it for a different purpose, but did not have any issues with it.
CFX96 from Biorad provides five channel detection and therefore you can multiplex upto five fluorescent dyes. It also provides a thermal gradient feature required for PCR optimization. It also has an additional FRET channel. You can also look for the QuantStudio 5 from Thermofisher Scientific which comes for a higher cost, providing 21 filter combinations for multiplexing upto 6 targets. Moreover, the QuantStudio 5 is also a faster machine due to its higher ramp rates PCR cycling can be completed in around 30 mins. QuantStudio 3 comprises of only 4 channels.
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