What's a fast + accurate way to ID SCN boundaries from frozen rat brain?

01 January 1970 1 2K Report

We had planned to Nissl stain 20 um sections and punch out SCN for RNA- and BS-seq, but I am worried about too many freeze-thaws causing degradation. Could anyone recommend a streamlined method/protocol for delineating SCN boundaries for tissue punching?

Thanks very much.

Mich

Mothana Ali Khalil

I hope to hear the answer

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