I need to know how to determine carotenoid content in extracted shrimp waste sample and hope to use extracts as food ingredient. but here unavailable the carotenoid standards to determine carotenoid content using spectrophotometer and HPLC
To determine the carotenoid content in extracted shrimp waste samples for potential use as a food ingredient without access to carotenoid standards, you can use alternative methods that do not rely on standards for quantification. Here are two approaches you can consider:
Spectrophotometric Method:
Procedure:
Prepare the extracted shrimp waste sample for analysis.
Measure the absorbance of the sample at specific wavelengths associated with carotenoids (e.g., 450 nm for astaxanthin).
Calculate the carotenoid content using the Beer-Lambert law, which relates absorbance to concentration.
HPLC Method:
Procedure:
Analyze the extracted sample using an HPLC system equipped with a UV-Vis detector.
Compare the chromatogram of the sample to known carotenoid standards to identify and quantify carotenoids present.
Use peak areas or peak heights to estimate the concentration of carotenoids in the sample.
Dear Abdelhak Maghchiche
you suggest "using the Beer-Lambert law, which relates absorbance to concentration." You need to know the extinction coefficient, which can be determined using the standard solution.
The same problem for HPLC.
Your post does not answer the title question.
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