Using 2% w/v Gelatin A and Gum acacia, 0.5gm crystalline drug and 1ml 10% tween 80.h20

So I adjust to ph to 3.8 and subjecting it to ice bath to reduce the temperature to 15C.

Repeat experiment twice:

I check the coacervate under microscope and

1) Replicate 1: I see spicky-like stuff sticking around it, and some floating around the solution.

2) Replicate 2: No spicky-like stuff. Surrounding clear

= Spicky stuff only appear after continuous agitation at 15C

I am guessing spicky-like stuff= probably gelatin+arabic. But why is it appearing so?

Is it because its been cool down too fast, as compare to replicate 2?

http://www.ncbi.nlm.nih.gov/pubmed/9743913