How can i experimentally validate the microRNA binding in CDS region of a gene?

I am using pMIR-Report for the checking the binding of microRNA on 3'UTR of a gene through luciferase assay. Can i use this same vector for checking the binding of microRNA on CDS as the binding...

10 November 2018 1,326 3 View

Can last 6 nucleotides be missed in insert in PGEMT vector in TA cloning ?

I have designed stem loop primers with last 6 nucleotide of gene of my interest and respective forward primers and universal reverse primers against stem loop primers. I got successful cloning and...

01 February 2018 4,319 9 View

Is it essential to characterise the sequence of novel microRNAs after bioinformatic analysis with mirdeep2 through experimental cloning strategy?

I need to experimentally validate the novel microRNAs in my patient cohorts. Before that is it necessary to do cloning of mature/precursor mir sequence followed by sanger. If so , Can anyone share...

09 October 2017 5,722 5 View

What should be the randfold value for predicting novel MicroRNAs ?

I am facing problem in selecting novel microRNAs from small RNA sequencing. mirdeep 2 software was used for prediction of novel microRNAs. Is it necessary to have high mirdeep2 score with high...

08 September 2017 6,551 4 View

How can we analyze/ study the effect of deep intronic variation using bioinformatic tool ?

31 December 2016 6,361 7 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View