Gurjeet Kaur

6 Questions 12 Answers 0 Followers

Questions related from Gurjeet Kaur

How can i experimentally validate the microRNA binding in CDS region of a gene?

I am using pMIR-Report for the checking the binding of microRNA on 3'UTR of a gene through luciferase assay. Can i use this same vector for checking the binding of microRNA on CDS as the binding...

11 November 2018 1,368 3 View

Can last 6 nucleotides be missed in insert in PGEMT vector in TA cloning ?

I have designed stem loop primers with last 6 nucleotide of gene of my interest and respective forward primers and universal reverse primers against stem loop primers. I got successful cloning and...

02 February 2018 4,354 9 View

Is it essential to characterise the sequence of novel microRNAs after bioinformatic analysis with mirdeep2 through experimental cloning strategy?

I need to experimentally validate the novel microRNAs in my patient cohorts. Before that is it necessary to do cloning of mature/precursor mir sequence followed by sanger. If so , Can anyone share...

10 October 2017 5,757 5 View

What should be the randfold value for predicting novel MicroRNAs ?

I am facing problem in selecting novel microRNAs from small RNA sequencing. mirdeep 2 software was used for prediction of novel microRNAs. Is it necessary to have high mirdeep2 score with high...

09 September 2017 6,603 4 View

Whats the exact function of sequence tagged sites in a gene ? Does the single nucleotide variations affect its function ?

I found single nucleotide change in sequence tagged site . though that region is not part of CDS but its the part of STS.

04 April 2017 3,105 2 View

How can we analyze/ study the effect of deep intronic variation using bioinformatic tool ?

The intronic variations , which i have found are not at exon/intron junctions.

01 January 2017 6,403 7 View