What's the difference between yeast plasmid pRS316 and pRS416?

26 April 2021 1 138 Report

I want to do a plasmid shuffling assay in saccharomyces cerevisiae YPH499， Which series of plasmids are suitable for YPH499, 3 or 4? Is there any difference between series 3 and 4? thanks very much

Michael J. Benedik

I think the only difference between pRS316 and pRS416 is that some restriction sites were modified so that they would be unique in pRS416. Some of the sites in the multicloning site were not unique in the 3 series and existed elsewhere on the plasmid.

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