Mingdong Liu

2 Questions 6 Answers 0 Followers

Questions related from Mingdong Liu

A question about yeast gene disruption?

Now I want to do a gene disruption in the yeast strain SEY6210. The mark gene was LEU2. Positive clones were identified by PCR (Primers were designed at both ends of the target gene). PCR results...

14 October 2021 2,604 11 View

What's the difference between yeast plasmid pRS316 and pRS416?

I want to do a plasmid shuffling assay in saccharomyces cerevisiae YPH499， Which series of plasmids are suitable for YPH499, 3 or 4? Is there any difference between series 3 and 4? thanks very much

26 April 2021 160 1 View