Parsing error in autodock vina?

I'm trying to use autodock vina, and I received the following error for the ligand file when running the configuration: "Parse error on line 26 in file "15.pdbqt": Unknown or inappropriate...

21 October 2020 7,245 1 View

Does anybody have any knowledge of "dry" microbiological decontamination methods for frozen fruit?

I am looking for data on the implementation of a microbiological reduction step for frozen fruit pieces. I have information on cold plasma/UV but am unable to locate much data on gaseous methods...

24 November 2019 6,131 8 View

Has anyone used the MinuteTM lysosome isolation kit to isolate lysosomes?

This seems to be the only kit (made by Invent Biotechnologies) that's based on a non-density gradient and ultracentrifugation method. Has anyone used it successfully to isolate lysomes from...

20 November 2019 7,210 3 View

Has anyone used the MinuteTM lysosome isolation kit to isolate lysosomes?

This seems to be the only kit (made by Invent Biotechnologies) that's based on a non-density gradient and ultracentrifugation method. Has anyone used it successfully to isolate lysomes from...

10 November 2019 9,907 3 View

Can tissues dissolve in formalin?

My question pertains to human heart tissue previously embedded and frozen in OCT. We have recently cut sections of varying thickness (100-1000 um) for processing and staining while free-floating....

21 October 2019 7,148 3 View

Do I treat Likert scale data (predictors) with continuous DV as parametric or non-parametric?

Hi there, I am carrying out a study on factors affecting electric vehicle adoption. I am using a likert scale format (from strongly disagree to strongly agree) for all of the predictor variable...

13 August 2019 258 3 View

Do I use multiple regression or hierarchy multiple regression?

My study is on electric vehicles and I want to test whether factors such as cost and range anxiety have significant effects and their degree of influence when analysing the intent to adopt. I am...

03 July 2019 9,547 10 View

Are there any drawbacks of using Peak Area instead of Peak Height to calculate ratio of alleles in fragment analysis?

I am setting up a fragment analysis assay in which I need to calculate the ratio of the two peaks. The protocol I have inherited suggests using Peak Height, and I see this used a lot generally for...

07 May 2019 9,369 1 View

RNA Free Energy?

If you have a validated RNA structure (with or without pseudoknots) can you back calculate a theoretical free energy for that structure? I know many programs that calculate a free energy and try...

20 January 2019 7,763 2 View

In your opinion, should ALL research articles be shared and accessed freely? Please give a reason for your answer (why)?

This will throw more light on the open access policy of publishing institutions

12 June 2018 7,439 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

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22 February 2021 9,932 11 View

How to locate and collect the pineal gland in the amphibian brain?

Our team has been trying to locate and collect the pineal gland in the American bullfrog's brain (Lithobates catesbeianus) for some time now. However, we don't have a standardized protocol for...

18 February 2021 3,453 4 View