Hi, how can I determine the amplicon size of my template with two primers from a gene construct (p-35S/nptII)? The FW primer targets one region (p-35S), and the RV primer targets another region (nptII). Because of this, I can't find the amplicon using BLAST. Could someone help me figure this out so I can calculate the number of copies?
FW p-35S: TAT CCT TCG CAA GAC CCT TCC RV nptII: GAT TGT CTG TTG TGC CCA GTC A
Péter Gyarmati
Do you have the full sequence of your gene construct? If so you can just search for the primer sequences.
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Kaylee Huijsers
I don’t know where I can find that. The gene construct is P-35S / nptII. Do you maybe have an idea?
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Péter Gyarmati
https://www.addgene.org/160646/sequences/
this one? otherwise check it with the company it was ordered it from/your PI may know it
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