Kaylee Huijsers

3 Questions 4 Answers 0 Followers

Questions related from Kaylee Huijsers

How can I determine the amplicon sequence when only using the FW primer, RV primer and probe for qPCR?

I’m working on a multiplex real-time PCR project where I need to amplify a combined region from the CPT2-CP4-epsps gene for the detection of GMOs. I have the following oligos: Forward Primer (F):...

09 May 2025 1,467 7 View

How to Internal Control Design for Multiplex qPCR?

I am developing a multiplex qPCR assay for GMO detection and am considering using an oligoset originally designed for the 23S rRNA gene to detect a synthetic DNA template as an internal control...

15 April 2025 7,275 2 View

Whats the amplicon size?

Hi, how can I determine the amplicon size of my template with two primers from a gene construct (p-35S/nptII)? The FW primer targets one region (p-35S), and the RV primer targets another region...

24 January 2025 2,657 3 View