What is a good marker for imaging mouse macrophages by immunofluorescence in FFPE lymph nodes?

I am new to using mice, and I would like recommendations for specifically staining most, if not all macrophage subsets in murine tissues, particularly in lymph nodes and spleen. My background is...

16 November 2020 3,412 3 View

What is the current status of EOS modelling in unconventional reservoirs, esp. those with uplift and secondary migration of high-maturity gas?

Equation of state (EOS) modelling in CONVENTIONAL reservoirs is well established and has been successfully employed in PVT studies of basins globally for many years. However, what is the current...

12 November 2020 5,153 1 View

Can I image AlexaFluor488-labeled proteins in fixed tissue?

I'm planning to administer an immunogen labeled with AlexaFluor-488 into macaques with the goal to image the immunogen in the draining lymph node after it is biopsied. I'm having trouble finding...

21 September 2020 7,156 3 View

How to calculate sample sizes for multiple logistic regression?

Dear friends, I am looking for a procedure to calculate sample sizes/evaluate the power in logistic regression models with 3+ predictors which are partly binary partly continuous variables. Can...

03 October 2019 5,336 5 View

How do I analyze Likert scale questions in my dataset?

Hi, I have a data set in SPSS with several Likert scale questions. I wanted to see if there are any differences with how males and females answer several satisfaction questions (e.g., satisfied...

19 August 2018 1,554 4 View

Does resistance have an upper cross sectional limit where the proportional definition of resistance and resistivity breaks down?

I have seen evidence of a lower limit of this equation, but want to determine the resistivity of a composite semiconductor that has a cross section on the order of 100's mm^2 and about 100 mm in...

28 November 2017 8,453 2 View

Is there an open data collection of news headlines?

Some of my colleagues are planning to analyse news headlines related to climate change. Does anyone know of a good source for historical news headlines as open data?

08 September 2017 8,320 4 View

In alkaline comet assay is it possible to use normal melting agarose diluted with milli-Q water, or you have to dilute it with TBE?

Our slides coated with NMA with TBE always get cloudy after drying. We are using alkaline comet assay to evaluate sperm DNA fragmentation.

11 December 2016 2,823 5 View

Is there neuroscientific work on the construction of causality/causation?

I am interested in literary plots and wonder whether there is neuroscientific research on how readers/viewers construe causality. If so - and if there is such an ability - how is it related to...

09 October 2016 1,974 2 View

Is there any relevance theoretical work that addresses the question of folk psychology?

This question is in connection with an article I am writing. Relevance theory seems potentially helpful for my argument, but I need to find work that explicitly addresses the link between the...

28 September 2015 8,493 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View