I have optima polar star plate reader and I want to measure the fluorescence at Ex390/Em 510 and Ex488/Em510 but the machine does not has the specific wavelength filters. Excitation wavelengths are 595, 584, 370, 570,485,420 and emission wavelengths are 450, 590, 520,620,405, 460 etc.
Can I use any of the combination of Ex/Em from above wavelengths?
Thank you.