How to add activated carbon and yeast extract in my data inventory for Life cycle analysis using SimaPro?

I want to carry out life cycle analysis of a process using SimaPro software. My process involves the use of both yeast extract as a nutrient for fermentation and activated carbon for a...

03 April 2019 5,909 1 View

PCR amplification problem?

I am trying to amplify a 6 kb fargment from a plasmid of size 6.9 Kb. I am getting my desired band alongwith some background shear. Could anyone tell me how to solve the problem. I am attaching a...

04 May 2016 5,503 6 View

How to measure NADPH concentration levels inside a cell?

COuld anyone suggest me if there is a standard protocol to measure NADPH concentration levels inside the cell?

03 April 2016 443 1 View

Can anyone troubleshoot my transformation problem?

03 April 2016 4,545 1 View

SOlution to real time PCR problem?

02 March 2016 1,470 7 View

Anyone familiar with assembling in velvet queries?

I am trying to carry out de novo assembly of a genome of interest. I have some queries regarding operation of velvet tool. When the k mers are selected and hash table is made, does velvet know...

03 April 2015 1,658 1 View

Any suggestions on whole genome de novo assembly by velvet using paired end reads?

02 March 2015 4,719 5 View

How do I solve my protein purification problem?

I have theoretical PI of my protein from sequence data which is 5.59. I have tried anion exchanger (DEAE-macrosep) starting with buffer at pH 8 and then reducing it down to 6 followed by NaCl...

01 February 2015 3,930 16 View

Primer and probe design for gene overexpression?

I want to check expression level of a gene of interest in an organism. I am new to real time-PCR. Could one kindly guide me how to proceed. I already have designed primers for amplification of the...

11 December 2014 8,058 2 View

Can anyone provide me with a protocol for RT-PCR?

Can anyone provide me link of any paper to carry out RT-PCR for testing gene overexpression? I need the full protocol.

11 December 2014 5,679 4 View

Timeout error!! Processor in the loop (PIL) using STM32F4 Matlab 2018a?

Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...

01 March 2021 2,327 1 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

No title

I am trying to find how to know the density needed for a pva electrospinning in a 0.6mm neddle, the electrospinning will be from top to bottom and the problem I really have is the solution because...

25 February 2021 2,615 3 View

How Can I clain a removal of a co-author?

I would like to make a correction in a co-author in your paper which is wrong. This name is also in other papers of Kenton Ko. Zey Ko is not a Co-author . The correct name is Zdenka...

24 February 2021 3,476 4 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

How the values of errors can be calculated for thermodynamic data?

I have calculated thermodynamic parameters for surfactant solutions in aqueous and mixed alcoholic solvents. I want to calculate the value of errors in the data and represent it in the table along...

22 February 2021 239 2 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View

Difficulty in debugging code to correct error - TypeError: '<' not supported ?

I am trying with a Unet code ontained from net ...in tensorflow. When trying to predict the segmentation result with the trained and saved model with metric " iou" using "CustomObjectScope"...

22 February 2021 5,254 1 View

How to perform Haldane correction on SPSS?

I was wondering if anyone knew how to perform the Haldane correction for SPSS (this is the definition of the correction -...

21 February 2021 2,882 1 View