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Questions related from Diptarka Dasgupta
I want to carry out life cycle analysis of a process using SimaPro software. My process involves the use of both yeast extract as a nutrient for fermentation and activated carbon for a...
04 April 2019 5,987 1 View
I have carried out a genetic modification where the functional URA3 gene in the yeast has been partially replaced by Ura3-KanMX-Ura3 disruption cassette. I want to know what would be the exact...
12 December 2018 5,008 4 View
I am trying to amplify a 6 kb fargment from a plasmid of size 6.9 Kb. I am getting my desired band alongwith some background shear. Could anyone tell me how to solve the problem. I am attaching a...
05 May 2016 5,543 6 View
Dear all, I am trying to transform a URA3- mutant of K. marxianus strain with a plasmid containing Sacch URA gene. I checked whether the cells were URA3- or not by plating them on FOA....
04 April 2016 4,588 1 View
COuld anyone suggest me if there is a standard protocol to measure NADPH concentration levels inside the cell?
04 April 2016 481 1 View
Dear all, I am facing a problem with my real time PCR experiment. I had isolated RNA from a strain of interest (RNA conc. 25 ng/uL) and carried out cDNA synthesis with 8uL of the RNA...
03 March 2016 1,520 7 View
I am trying to carry out de novo assembly of a genome of interest. I have some queries regarding operation of velvet tool. When the k mers are selected and hash table is made, does velvet know...
04 April 2015 1,697 1 View
I have assembled genome of my strain of interest. I have the assembly stats in a log file, stats.txt and contig.fa file. I wish to know the stats such as maximum contig size, minimum, average...
03 March 2015 4,758 5 View
I have theoretical PI of my protein from sequence data which is 5.59. I have tried anion exchanger (DEAE-macrosep) starting with buffer at pH 8 and then reducing it down to 6 followed by NaCl...
02 February 2015 3,969 16 View
Can anyone provide me link of any paper to carry out RT-PCR for testing gene overexpression? I need the full protocol.
12 December 2014 5,725 4 View
I want to check expression level of a gene of interest in an organism. I am new to real time-PCR. Could one kindly guide me how to proceed. I already have designed primers for amplification of the...
12 December 2014 8,100 2 View
I have modeled 3D structure of my protein and want to immobilize it on a catalytic surface/support. Is there any such tool in discovery studio/material studio that would enable me to carry out the...
10 October 2014 482 3 View
Can anyone suggest me a good book on protein modelling which covers all the aspects starting with homology modelling to principals of solvation, electrostatics, molecular dynamics, docking etc?
09 September 2014 208 1 View
