What would be the correct genotype representation of my Ura3 mutant?

I have carried out a genetic modification where the functional URA3 gene in the yeast has been partially replaced by Ura3-KanMX-Ura3 disruption cassette. I want to know what would be the exact...

11 December 2018 4,962 4 View

PCR amplification problem?

I am trying to amplify a 6 kb fargment from a plasmid of size 6.9 Kb. I am getting my desired band alongwith some background shear. Could anyone tell me how to solve the problem. I am attaching a...

04 May 2016 5,503 6 View

How to measure NADPH concentration levels inside a cell?

COuld anyone suggest me if there is a standard protocol to measure NADPH concentration levels inside the cell?

03 April 2016 443 1 View

Can anyone troubleshoot my transformation problem?

03 April 2016 4,545 1 View

SOlution to real time PCR problem?

02 March 2016 1,470 7 View

Anyone familiar with assembling in velvet queries?

I am trying to carry out de novo assembly of a genome of interest. I have some queries regarding operation of velvet tool. When the k mers are selected and hash table is made, does velvet know...

03 April 2015 1,658 1 View

Any suggestions on whole genome de novo assembly by velvet using paired end reads?

02 March 2015 4,719 5 View

How do I solve my protein purification problem?

I have theoretical PI of my protein from sequence data which is 5.59. I have tried anion exchanger (DEAE-macrosep) starting with buffer at pH 8 and then reducing it down to 6 followed by NaCl...

01 February 2015 3,930 16 View

Primer and probe design for gene overexpression?

I want to check expression level of a gene of interest in an organism. I am new to real time-PCR. Could one kindly guide me how to proceed. I already have designed primers for amplification of the...

11 December 2014 8,058 2 View

Can anyone provide me with a protocol for RT-PCR?

Can anyone provide me link of any paper to carry out RT-PCR for testing gene overexpression? I need the full protocol.

11 December 2014 5,679 4 View

How to quantify carbon deposition on CuZnAl catalyst?

Dear All, I want to quantify carbon deposition on the surface of the CuZnAl catalyst. I have tried thermogravimetric analysis, but I've got weight gain as the active Cu- get oxidised. Does anyone...

01 March 2021 1,907 3 View

Why Trivalent chromium start precipitating above PH 5 ? Moreover discuss difference between adsorption of chromium and precipitation of chromium ?

I am a student of MS Environmental Engineering and started my research phase. I am working on metal removal ( chromium iii ) by using activated carbon prepared from the agricultural feedstock. So...

01 March 2021 5,475 2 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

What are the methods used for CNT..?

Respected Professor's i hope you are doing well and happy. i need some information about carbon Nanotubes. before blending the CNT with the matrix the CNT has been introducing in some treatments....

24 February 2021 5,383 5 View

Have you ever experiencing air vent trouble in fermentation process?

Dear all, My research group is currently working with a 12-litre fermenter. Somehow, if we do the fermentation for more than 20 hours, the process will be more prone to contamination. Our current...

23 February 2021 1,908 2 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

What are some underexplored theories and concepts in Activated Carbon as A Water Filter?

Hi, me and my team mates are currently planning to pursue the topic of Activated Carbon from Coconut as a Water Filter. And we would like to ask for your suggestions and knowledge on what specific...

22 February 2021 6,569 3 View

What are some underexplored theories and concepts in Activated Carbon as A Water Filter?

Hi, me and my team mates are currently planning to pursue the topic of Activated Carbon from Coconut as a Water Filter. And we would like to ask for your suggestions and knowledge on what specific...

21 February 2021 7,561 4 View

What properties of water shall be analyzed and evaluated to test the effectiveness of activated carbon?

I would like to ask what other properties of water (pH and such) shall be analyzed and evaluated to test the effectiveness of activated carbon from filtering the contaminants present in the water?

20 February 2021 9,422 8 View