How to add activated carbon and yeast extract in my data inventory for Life cycle analysis using SimaPro?

I want to carry out life cycle analysis of a process using SimaPro software. My process involves the use of both yeast extract as a nutrient for fermentation and activated carbon for a...

03 April 2019 5,909 1 View

What would be the correct genotype representation of my Ura3 mutant?

I have carried out a genetic modification where the functional URA3 gene in the yeast has been partially replaced by Ura3-KanMX-Ura3 disruption cassette. I want to know what would be the exact...

11 December 2018 4,962 4 View

PCR amplification problem?

I am trying to amplify a 6 kb fargment from a plasmid of size 6.9 Kb. I am getting my desired band alongwith some background shear. Could anyone tell me how to solve the problem. I am attaching a...

04 May 2016 5,503 6 View

Can anyone troubleshoot my transformation problem?

03 April 2016 4,545 1 View

SOlution to real time PCR problem?

02 March 2016 1,470 7 View

Anyone familiar with assembling in velvet queries?

I am trying to carry out de novo assembly of a genome of interest. I have some queries regarding operation of velvet tool. When the k mers are selected and hash table is made, does velvet know...

03 April 2015 1,658 1 View

Any suggestions on whole genome de novo assembly by velvet using paired end reads?

02 March 2015 4,719 5 View

How do I solve my protein purification problem?

I have theoretical PI of my protein from sequence data which is 5.59. I have tried anion exchanger (DEAE-macrosep) starting with buffer at pH 8 and then reducing it down to 6 followed by NaCl...

01 February 2015 3,930 16 View

Primer and probe design for gene overexpression?

I want to check expression level of a gene of interest in an organism. I am new to real time-PCR. Could one kindly guide me how to proceed. I already have designed primers for amplification of the...

11 December 2014 8,058 2 View

Can anyone provide me with a protocol for RT-PCR?

Can anyone provide me link of any paper to carry out RT-PCR for testing gene overexpression? I need the full protocol.

11 December 2014 5,679 4 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to Eliminate cosine error and abbe error in LASER measurement system(for example Rhenishaw XL80)?

I like to do project in laser measurement system. My objective is to eliminate or reduce the cosine error, abbe error in Laser measurement system. I have Rhenishaw XL80 system. Please tell the...

28 February 2021 4,890 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View