04 April 2016 1 525 Report

COuld anyone suggest me if there is a standard protocol to measure NADPH concentration levels inside the cell?

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How to add activated carbon and yeast extract in my data inventory for Life cycle analysis using SimaPro?

I want to carry out life cycle analysis of a process using SimaPro software. My process involves the use of both yeast extract as a nutrient for fermentation and activated carbon for a...

03 April 2019 6,025 1 View

What would be the correct genotype representation of my Ura3 mutant?

I have carried out a genetic modification where the functional URA3 gene in the yeast has been partially replaced by Ura3-KanMX-Ura3 disruption cassette. I want to know what would be the exact...

11 December 2018 5,042 4 View

PCR amplification problem?

I am trying to amplify a 6 kb fargment from a plasmid of size 6.9 Kb. I am getting my desired band alongwith some background shear. Could anyone tell me how to solve the problem. I am attaching a...

04 May 2016 5,563 6 View

Can anyone troubleshoot my transformation problem?

Dear all,             I am trying to transform a URA3- mutant of K. marxianus strain with a plasmid containing Sacch URA gene. I checked whether the cells were URA3- or not by plating them on FOA....

03 April 2016 4,620 1 View

SOlution to real time PCR problem?

Dear all,              I am facing a problem with my real time PCR experiment. I had isolated RNA from a strain of interest (RNA conc. 25 ng/uL) and carried out cDNA synthesis with 8uL of the RNA...

02 March 2016 1,556 7 View

Anyone familiar with assembling in velvet queries?

I am trying to carry out de novo assembly of a genome of interest. I have some queries regarding operation of velvet tool. When the k mers are selected and hash table is made, does velvet know...

03 April 2015 1,725 1 View

Any suggestions on whole genome de novo assembly by velvet using paired end reads?

I have assembled genome of my strain of interest. I have the  assembly stats in a log file, stats.txt and contig.fa file. I wish to know the stats such as maximum contig size, minimum, average...

02 March 2015 4,786 5 View

How do I solve my protein purification problem?

I have theoretical PI of my protein from sequence data which is 5.59. I have tried anion exchanger (DEAE-macrosep) starting with buffer at pH 8 and then reducing it down to 6 followed by NaCl...

01 February 2015 3,994 16 View

Primer and probe design for gene overexpression?

I want to check expression level of a gene of interest in an organism. I am new to real time-PCR. Could one kindly guide me how to proceed. I already have designed primers for amplification of the...

11 December 2014 8,121 2 View

Can anyone provide me with a protocol for RT-PCR?

Can anyone provide me link of any paper to carry out RT-PCR for testing gene overexpression? I need the full protocol.

11 December 2014 5,748 4 View

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