I have a 30 a.a. length lyophilized peptide which has a gradient of hydrophobicity along the helix axis and the net charge of -1
Which option is more important to determine the best solvent/buffer for such a peptide to be dissolved in? The net charge or the hydrophobicity gradient?
Hi Azra,
I would recommend HFIP (Hexafluoroisopropanol). If it doesn't work , you can go for formic acid.
Thank you for your answer Mazda, but could you please add why you have recommended each of these solvents?
The main reason is the peptides that I've worked on that have similar gradient hydrophobicity from N- toward to C- terminus ( and these 2 solvents worked well) . However, HFIP is a good solvent for peptides with helix.
I recommend HFIP (Hexafluoroisopropanol). If it doesn't work , you can go for formic acid.
HFIP is an excellent first choice, if you want you can always use DMSO that will dissolve the peptide for sure!
- Badges
- Science topic