I'm staining frozen sections of the human lung and would need a good marker to identify epithelial cells in immunofluorescence. EpCAM apparently is not expressed enough in the airway epithelium, what could I use instead?
What type of cells you want to identify?
a cytokeratin 19 antibody is a good marker for bronchial cells.
DClamp allows to visualize all type II pneumocytes.
Regarding the dendritic cells, there are several markers specific for each family of DC:
Langerin or ASGPR for the epithelial tissue.
DCsign or MR, for the alveolar tissue.
Thank you for your answer! I want to visualize peripheral bronchial epithelial cells so the cytokeratin 19 would work for me, right?
Keratin 19 for sure! EpCAM is not expressed in mucous squamous epithelia of the airway.
Hello Eliisa. I'm trying to find out more about EpCAM expression in the airway epithelium. Do you have any useful references or is what you know based on experimental observation? Thank you.
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