Eliisa Kekäläinen

3 Questions 6 Answers 0 Followers

Questions related from Eliisa Kekäläinen

Does anyone have experience using single-cell Western blotting?

I'm interested in the Milo by Protein Simple. I would appreciate any practical experiences, will it work? We would like to test targets found with single-cell RNASeq.

25 April 2017 976 1 View

What are the best robust mitogens for polyclonal T cells stimulation in vitro?

We are currently using the FASCIA method for in vitro stimulation of whole blood (ref: Marits P. et al. Clin Immunol PubMed ID 24909732). We use PHA, PWM and ConA for stimulation but I don't...

04 July 2016 8,103 7 View

What would be the best marker in IHC/IF for bronchial epithelial cells?

I'm staining frozen sections of the human lung and would need a good marker to identify epithelial cells in immunofluorescence. EpCAM apparently is not expressed enough in the airway epithelium,...

03 February 2014 6,806 5 View