Chaitra Hiremath

2 Questions 5 Answers 0 Followers

Questions related from Chaitra Hiremath

Non heated protein sample on blots is giving non specific binding with Rabbit antibody but gives specific bands with Mouse antibody. Why?

I am working with A.thaliana protein. I have a heat sensitive GFP tag. So I cannot heat my samples before loading onto the gel for western blotting. I Incubated the blot with Rabbit-AntiGFP...

03 April 2023 9,588 6 View

What would be the best extraction buffer for membrane bound plant proteins?

I am trying to extract my membrane bound plant protein with different extraction buffers but i am unable to extract most of it. So can anyone suggest a extraction buffer ?

21 May 2021 3,307 3 View