i am using SYBR Green master mix
i have to study the awareness and adoption level of cloud computing in a district of India. i also want to use association among demographic variables like gender, age, education, income etc and...
02 August 2024 2,420 3 View
Steel processing industry releases waste water, which contains 20% of Iron with 2 pH. can you suggest any low-cost technique to separate or recover anyone, either acid or Fe from the waste water, pls
24 April 2024 5,636 6 View
Our samples had been analysed by Mothur + Silva 119 reference DB around 2015. When analysing the same samples in 2023 with CLC genomics workbench and SILVA 119 DB, the results are strikingly...
21 March 2024 991 1 View
I have made a natural product loaded nanogels. Through MTT assay I have found out the drug dose but am not getting how to treat the cell further with the formulations
04 March 2024 5,389 1 View
For a better teaching in the class.
19 February 2024 274 1 View
Hi all, I'm trying to plot Free Energy Landscapes from Gromacs after using the gmx sham function. After using the command: gmx sham -f PCAplot2d.xvg -notime -ls gibbs1-2.xpm (where PCAplot2d.xvg...
06 January 2024 6,496 0 View
I have cloned my genes in a CMV vector and transfected them in HEK293 cells. After harvesting the cells, the expression level at the transcriptional level is increased compared to the control...
23 December 2023 2,507 10 View
During storage of Ethanol (USP Grage) after filtration through 5-micron filter membrane. Particulate matter observed during storage. For that, we need to investigate the reason. Is any science...
26 November 2023 2,540 3 View
In one of our pregnane ring structure related product (Drospirenone), we are facing one impurity (i.e. 6-Methyl Drospirenone impurity) problem. The observed level of impurity is about 1% on USP...
25 November 2023 5,102 16 View
how can i form the schematic diagram of g quadruplex as shown in figure?
24 November 2023 1,851 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View
