We are sampling on the environment for fungi growing on trees.
We would like to enrich the cultures. What would you suggest. Using starch media, which is easily available for fungi and bacteria. Or using cutting from branches derived from the same sampling trees as carbon source, that would select the fungi able to degrade that biomass. But perhaps the enrichment process will be slower than the one using starch?
enrichment means if it is a soil sample-you have to prepare soil extract.
If it is sediment you hhave to prepare sediment extract.
If it is bark of a plant : you have to prepare bark extract
and so on : you have to enrich the media withis natural substarates.
Using stach media you will only able to isolates microbes using starch as carbon source. This might be fungi you desired growing on tree or other fungi which might be present in tree but not growing there.
But why use starch. Use media containing lignin/ cellulose/pectin/hemicellulose for better isolation of desired fungi. But yes these media might also grow fungi on tree which are present but not growing.
I doubt that using branches you will get growth. Using extracts from branch might lead to growth of fungus but it might be slow. Also lab condition might not lead all fungus to grow.
So i believe use synthetic media using lignin/ cellulose/pectin/hemicellulose as carbon source along with media containing plant extracts (pretreated/organic solvent extracted) and compare the colonies.
Dear colleague
1. first supplement the medium with extract of the substrate(the sample from where you would like to isolate trhe fungi). If it is soil add 20% soil extract to the medium.
2. or simply you can use 1% water agar
3. Best would be to use potato infusion agar as the ist substrate
enrichment means if it is a soil sample-you have to prepare soil extract.
If it is sediment you hhave to prepare sediment extract.
If it is bark of a plant : you have to prepare bark extract
and so on : you have to enrich the media withis natural substarates.
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