Is Analysis of Similarity (ANOSIM) sensitive to differences in sample size?

Hi there. I want to compare 10 small forest landscapes on which different harvest designs were applied and see how much their attributes (structure, species, soil moisture...) differ. ANOSIM seems...

29 March 2020 5,826 4 View

Is there a way to download all molecules from a published research paper?

Hello, Is there a (preferably free) way to download all molecules (SMILES or SDF) from a published scientific article? Like searching pubchem or chembl or ZINC via DOI or so? I would like to...

05 January 2020 7,542 3 View

How to add a journal ?

Hello, is it possible to add a journal ? (Les Cahiers de Neuropsychologie Clinique) Some of my references can't be complete. Thank you.

08 October 2019 8,622 3 View

Why my target protein shows two elution peaks in the chromatogram during Ni-NTA?

I obtained two peaks during the affinity chrokatography which are next to each other. After I run SDS-PAGE, both peaks are my target protein. I am curious about the reason behind that, can anyone...

28 August 2019 3,520 3 View

Is there any possibilities that the salt crystal traps IZIT dye?

Is there any possibilities that the salt crystal traps IZIT dye? Besides, is there any possibilities that the protein crystal does not traps the IZIT dye? Thank you.

10 April 2019 7,843 1 View

What would be an appropriate method to compute the watershed of a lake?

Hi there, I'm wondering what would be the best approach to create the watershed of a lake. I see two solutions here: - Creating the watersheds of all tributaries of the lake and aggregate them,...

02 March 2019 2,527 3 View

Why thrombin doesn't show in SDS-PAGE after thrombin cleavage?

I have incubated my protein with thrombin, and it has successfully cleaved the tag from my protein. I was thinking that the thrombin should have show in the SDS-PAGE as well, but I didn't see any...

28 February 2019 10,006 10 View

Is surface water supply a hydrologic service, a forest service, or a watershed service?

There is no doubt that the access to surface freshwater is an ecosystem service. However, I feel that the many publications on ecosystem services and disservices with respect to the water supply...

07 January 2019 4,166 13 View

What are your tips for obtaining maximum resolution on an agarose gel?

I want to resolve very small deletions on agarose gel. My fragments are ~ 200 bp. What are your tips to get the best possible resolution? Here is what I have already gathered around: - 4%...

18 November 2018 5,740 6 View

Will the band of plasmid different from the expected base pair?

My plasmid size is ~6000bp, when I extracted the plasmid and run it in 1% agarose, it shows one band above 1kb, one band in between 1kb and 7000bp, and one band in between 5200bp - 4000bp. There...

31 October 2018 7,065 5 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

The peptide synthesized with CTC resin have mass more (+68 Da) than the desired mass of the synthesized peptide. What could be the reason?

I am synthesizing 7 amino acid long peptide sequence with CTC resin, 50oC coupling for 10 min. On cleavage (TFA/TIPS/Water 95:2.5:2.5) I got peptide with good yield. However, It has 68 Da more...

30 January 2021 8,483 3 View

How long does the peptide cleavage cocktail last before it gets expired?

The cleavage cocktail is composed of Phenol/thioanisole/water/TFA/triisopropylsilane. I only prepare 10 mL cocktail per peptide but can the activity of the cocktail prepared at 100 mL last for a week?

10 January 2021 7,424 3 View

What buffer to use for biotinylation as alternative to PBS?

I'm looking to biotinylate a protein that precipitates in PBS. The recommended reconstitution buffer for the protein is urea/Tris buffer, pH 7,5 (7 M urea, 5 mM EDTA, 15 mM mercaptoethanol, 20 mM...

04 January 2021 7,681 5 View

I would like to ask if anyone among you knows what is the ideal concentration of TFA to use at a chemical synthesis of peptides?

During a chemical synthesis of peptides by the F-moc method, the following happened: all the initial steps of the synthesis occurred as planned, but in the cleavage, TFA is used. In the lab, there...

02 November 2020 2,997 8 View

Why the expressed protein band Molecular Weight (MW) is lower than expectation?

I tried to express my protein in BL21(DE3) bacteria. The desired protein MW is 65.5 KDa and I know my protein will be in an insoluble form (inclusion body). I separate soluble and insoluble...

20 October 2020 3,975 8 View

How to cut the thrombin cleavage site if it is hindered by hydrophobic region?

I'm currently doing protein purification. my protein size is around 42kDa, however, my protein is attached with fusion tag which are Trx-tag and His-tag at the N terminal. I did optimize my buffer...

12 October 2020 2,083 3 View

How to purify a recombinant protein from Ecoli to reach a cell culture purpose?

05 October 2020 502 7 View