Astelia Francois

9 Questions 5 Answers 0 Followers

Questions related from Astelia Francois

What antibiotics is preferrable to inhibit bacterial growth in Fusarium culture?

Hi everyone. I would like to grow Fusarium oxysporum f.sp cubense TR4 on PDA media. I planned to add antibiotics to prevent bacterial contamination. So far as I searched, most people recommended...

18 July 2023 1,568 2 View

How to convert total protein concentration from mg/ml to molarity?

Hi everyone. I have protein with concentration of 0.6 mg/mL The total volume of my protein is 4 mL. Protein size is ~18 kDa How can I convert my total protein concentration to micro...

11 July 2023 8,638 8 View

Why the "Spot search fail" message appeared when I am trying to index X-ray diffraction data?

I am processing the X-ray diffraction data with imosflm. However, when I try to do indexing, an message saying "Blank image? Check image carefully." When I checked the images, they are all with...

06 September 2019 4,910 0 View

Will the use of tryptone and yeast extract in autoinduction media produce protein that is problematic for isotope-labeling?

I have went through some of the publications about the overexpression of protein for NMR studies. They had mentioned that the minimal media and autoinduction media can be used for producing...

10 May 2019 4,568 3 View

If my overexpression and purification steps carrying the metal ions that bind to my enzyme, will the ions show in the diffracted image of my protein?

It has been proposed that the metal ion will bind to the core structure of my protein, previous work had added this metal ion during the overexpression and purification procedures for the...

03 January 2019 8,075 2 View

What will happen if I incubate protein with thrombin for more than 18 hours?

I have looked through several protocols on thrombin cleavage, and all of them incubate the protein with thrombin for 16 - 18 hours. However, may I know what will happen to my protein if it is...

21 November 2018 6,242 3 View

Should I exclude the eliminated sequence from my target if I have eliminated some amino acid residues in my predicted model?

Since I have eliminated some amino acid residues in my predicted model, when I want to obtain the estimated properties such as molecular weight, theoretical pI, aliphatic index and so on, should I...

02 February 2018 3,323 2 View

Can somebody please explain the difference between the advanced modeling and loop refinement in MODELLER?

After I got my model with basic modeling in MODELLER, a long 'hanging' strand with 31 amino acids was recognized. So I went to advanced modeling and remodeled my protein with multiple templates. I...

31 January 2018 7,447 3 View

Template selection in MODELLER vs BLAST, what are the differences?

I am modeling my protein with MODELLER, and I referred to both MODELLER official site and youtube for the process. From MODELLER site, the structure used for template selection are generated in...

31 January 2018 7,182 3 View