I want to check pattern of protein expression in pancreatic B-cells Vs other pancreatic cells. Can someone let me know what will be best method to differentiate between pancreatic B-cells and other non-B-cells?
Two papers show "Isolation of mouse pancreatic alpha, beta, duct and acinar populations with cell surface markers" and "A novel method for the detection of viable human pancreatic beta cells by flow cytometry using fluorophores that selectively detect labile zinc, mitochondrial membrane potential and protein thiols". They showed that TTR and DPPIV are primarily expressed in alpha cells whereas DGKB and GPM6A have a beta cell specific expression profile. The combination of FluoZin-3, TMRE, and mBcl was sufficient and necessary for the determination of the viability and function of beta cells. Moreover, examination of proinsulin in the Golgi is another way to know whether beta-cells are actively synthesizing the hormone. This way might also work in paraffin-embedded sections. The websites are for your reference.
http://www.ncbi.nlm.nih.gov/pubmed/21539888
http://www.ncbi.nlm.nih.gov/pubmed/18412218
Beta cells will stain for insulin by IHC. Alpha cells will stain with glucagon, Delta cells with somatostatin. There are also gamma cells and epsilon cells, but I don't have experience staining those.
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