What was the concentration of Fe/Mn used for sinking in this experiment?

More Fatemah Allam's questions See All

What is the explanation of broad peak in cyclic voltammetric curve?

What is the difference between broad and sharp cyclic volatmmetric curves? and what are the reasons for broad curve? especially when analyzing carbon material?

01 February 2019 9,931 1 View

Is it important to add buffer in the cathode chamber of MFC?

Can I use distilled water as catholyte in MFC? or if I use Potassium ferrocyanide, can I dissolve it in distilled water instead of buffer solution?

11 December 2018 1,554 0 View

The llight produced from bioluminescent bacteria, is it able to stimulate photosynthesis in other nearby bacteria and other photosynthetic organisms?

Light produced by bioluminescent microorganisms is composed of photons, are they able to stimulate photosynthesis in other marine organisms? Thsnk you.

03 April 2018 3,443 0 View

What is more accurate to study the effect of pH on bacterial activities, preparing different buffers at different pHs, or adjusting the medium pH?

I am preparing to study the effect of pH on bacterial activities, what is the perfect way for that: 1. Using different buffers and adding the medium components? or 2. Adjusting the medium pH by...

02 March 2018 4,134 2 View

How can I write in my thesis that a Pseudomonas isolate is pyocyanin producer?

Is it enough and acceptable to mention that the observation of fluorescent color is a clue that my strain is pyocyanin producer? Thanks

11 December 2017 9,069 0 View

How can I get the heat of combustion in Energy Efficiency equation for MFC?

In the Energy efficiency equation, how can I get the heat of combustion? Thanks.

10 November 2017 5,435 1 View

What is the "Residual curve" regarding the polynomial fitting?

When I run the polynomial fitting on my data, a curve named "Residual curve"b appear after calculation, what is the meaning of that curve? and is it acceptable to use it instead of the fitting...

10 November 2017 1,464 0 View

How can I calculate number of electrons involved in electrochemical reduction of oxygen?

I am working in Microbial fuel Cell (MFC) and want to calculate number of electrons involved in reduction of oxygen. Thanks

10 November 2017 8,602 0 View

How can I obtain slope of any curve from Origin 6.0 or 8.0 software?

How can I use origin in getting the slope of any curve? Thanks.

10 November 2017 5,906 0 View

What is the fastest and accurate method for extraction and identification of polysaccharide, pyocyanin, and rhamnolipid from culture of Pseudomonas?

Can you help me getting method (s) for rapid extraction and identification of these three Pseudomonas products? Thank you.

10 November 2017 2,250 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Maohong Fan

Dr. Allam,

You can use ICP-MS to measure the concentrations of Fe and Mn in the solution.

Fatemah Allam

Dr. Maohong Fan,

Thanks for your answer, but I ask about the preparation method, the paper does not mention the exact concentration of Fe/Mn in the experiment.

Paper title: "Power generation from a biocathode microbial fuel cell biocatalyzed by ferro/manganese-oxidizing bacteria"