I am working on enhancing brewers yeast viability, and I am using Liquid Sabouraud media and liquid Saccharomyces cerevisiae. Initially, I made a single serial dilution and inoculated 1ml of the diluent. I would like to know what is the best yeast cell count/density to use and also the best volume to use for the inoculation. Should I dilute first (and how many times) before inoculating or should I add the yeast directly to the media?
the objective is to see how an extract affects yeast growth in a culture
I was concerned if the inoculum will be too small or too big, and thereby hampering how they use up the substrates.
A loop of each isolate utilizedto inoculate a 100ml of autoclaved extract of yeast, peptone, dextrose(YPD) broth
inerlenmeyer flasks(250)ml and incubated for 24 hours at 30°C, 120rpm, subsequently, transferredfrom each
yeast cells suspension 10ml then addto 125ml of broth which composed of10g/L peptone,6g/L yeast extract,
6g/L malt extract and 2g/L glucose with adjusted pH 5.0.
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