How to quantify total protein in crooked western lanes?

I've been using Revert700 total protein stain to detect total protein in my western lanes. While this stain is robust & reversible, I have trouble quantifying in Image Studio Lite (LiCor) and...

05 December 2020 9,014 3 View

Need help plotting long lists of data in Graphpad Prism -?

Hi, I am generating long lists of per-cell data that I'd like to visualize as dot plots per condition. The problem is that my conditions are defined by letters rather than numbers. Or sometimes I...

11 November 2020 6,707 1 View

Elisa kit recommendations for CD3 detection from frozen spleen samples?

Hi, I have some flash-frozen mouse spleens (in the -80C) that I'd like to analyze for CD3, preferably using an Elisa kit to reduce time. I've been searching various sites, but not a lot of options...

22 October 2020 8,727 2 View

What is the best ethanol concentration for gracilaria sp?

So I use Gracilaria sp. for my research. I have tried to use 70% ethanol but I didnt get phytochem compounds. So I'm thinking to try again but I'm still confused,should i use 80% or 96%? I read...

04 June 2020 2,792 4 View

How to resolve CPCe run-time error "9"?

Hi When trying to export the analysis I have done of my images from CPCe I get the following error message which says "Run-time error "9": Subscript out of range". I have uploaded a screenshot...

30 April 2020 1,972 1 View

Is this yeast contamination in primary glial cell culture?

This is a primary glial culture (taken from pups @PD2). Picture taken at 10DIV. Astrocyte layer is completely confluent. We are actually after the microglial layer underneath the astrocytes...

03 April 2020 8,938 1 View

Protease being degraded?

I am working with a serine protease. After purification, I ran a degradation assay with casein, and it appeared to work great twice in a row (first picture representative of both attempts, first...

03 April 2020 2,582 1 View

Experience in cell culture?

...

03 December 2019 1,719 6 View

Can semi-supervised machine learning be used when only a proportion of classes are defined?

Hi, I'm currently using Random Forests to classify acoustic data to corresponding bat species, however I was wondering if there is a way to also incorporate unsupervised cluster analysis into my...

13 March 2019 3,943 4 View

Which Music Composers Have Used Mathematical Formulae In Their Compositions To Represent Journeys?

I know lots of composers have created works around mathematical constructs such as the Fibonacci sequence. I would like to learn if any composers have used mathematical constructs in their music...

11 February 2019 1,153 6 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View