I think my question is pretty straight forward.
Thank you.
I am trying to plan an pulldown assay to find some prey protein for my bait protein. I am interested in using the flag tag because it is small and doesn't disturb structure and doesn't require...
11 December 2019 2,406 3 View
So I am a new PhD student but I have been doing good research since I have been an undergrad. I have scientific interest in a lot of things and firmly believe that "A change in perspective is...
10 November 2019 4,091 6 View
This is my understanding. If it is wrong, please correct me. So animals have an adaptive immune system where when they come into contact with a foreign substance they create antibodies to it and...
09 October 2018 1,547 13 View
So I know in molecular biology that the choice of primers is important. Some primers like to form primer dimers or trimers, especially if length of the primer in nucleotides is large. I have been...
09 October 2018 1,655 10 View
I recently conducted a coffee-cup calorimetry experiment in which we attempted to measure if the dissolution of inorganic crystals such as LiCl, NaCl, and SrCl were endothermic or exothermic based...
01 February 2018 4,761 3 View
My question is about polymerase chain reaction. From what I've learned the forward and reverse primers are important for directing the heat tolerant polymerase to amplify a genetic sequence, and...
11 December 2017 3,524 14 View
05 June 2017 1,587 6 View
03 April 2017 8,851 5 View
03 April 2017 8,786 3 View
02 March 2017 1,210 3 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
effect of double bond on the flexibility of polymers
07 April 2021 0 0 View
I am writing to request some information for making polymer color flakes. I would appreciate the help.
17 March 2021 0 0 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
