I would like to do docking for some organic compounds as anticancer. Experiment showed those compounds are active for MCF-7
Unfortunately, your question has no answer. There are dozens or hundreds of possible biomolecular targets in any line of cancer cells, from kinases to DNA and RNA polymerases, from topoisomerases to telomerase, from growth factors to receptiors, etc, etc. If you want to do that seriously, first you have to find e.g. what proteins are overexpressed in MCF-7, it is a very long work that will give you at least several dozens of potential targets. Well, you can find that in the literature. But I am sure that your compounds will be active against many other tumor cell lines as well - perhaps you have not tested them on several dozens of lines from some 200 types of cancer? But sometimes the structure of inhibitor can provide some idea about its mechanism of action, for example, if it contains typical DNA binding fragments.
Hi M.K., I agree with Igor in that there are no possible magic bullets that only target cancer cells without affecting normal cells.
Cancer is a multiplex disease that is not caused by easily identified targets. Every cancer is different in terms of how it is generated and how it spreads.
Ok, thanks a lot, IGor and Steingrimur. Our compounds are active for Breast cancer. Is it possible to find easily some enzymes for this study?
Hi M. K., I suggest that you take a step back and analyze whether your compounds are toxic to normal mammary epithelial cells before you invest time and effort to find a target on MCF7 cells.
ATCC has primary mammary epithelial cells. If your compounds kill both primary epithelial cells and the MCF7s, then they are probably not worth going after.
http://www.atcc.org/en/Products/Cells_and_Microorganisms/Human_Primary_Cells/Cell_Type/Epithelial_Cells/Mammary_Epithelial_Cells.aspx
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