What transfer conditions should I use to blot a membrane protein of size 82kDa?

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I have run a 2D gel to identify certain protein isoforms in rat urine. I did a western with a antibody that binds to all isoforms of the protein. The expected size is around 17kD-20kD., however...

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How do I remove phosphate group from a protein that has been phosphorylated on its Histidine residue?

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How can I solve my protein instability so it can be used in NMR and ITC studies?

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What are the membrane transfer conditions I should use during western blot if i have two proteins of varying sizes?

If i need to do western blot of two proteins of varying sizes separated in the same gel (one 55kDa and one 13kDa), what should be the membrane transfer conditions that I need to use? Is 70V for 2...

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Does the use of Phos-Tag give a high background?

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How to check protein stability?

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Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

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New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

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Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

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POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

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Will a 10xHis-tag prevent anti-MBP from binding to MBP?

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Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

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What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

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IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

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Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

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What is the best way to manage Western blots?

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