5'-ATGTTAATCCCTCATTGGAG-3' SEQUENCE I've to make amino acid sequence too. But when i'm trying to do that, last AG bps are being left out. What to do :(
Since 3 nucleotides are required to code for an amino acid, if there are one or two nt left at the 3'-end, they cannot be translated. The same goes for the 5'-end if you start at nt 2 or 3.
If you don't know which frame is the correct reading frame, you should try all three possibilities, starting at the first, second, and third nucleotides (counting from the 5' end). This will result in 3 different sequences of 6 residues, unless there is a stop codon.