What type of polyphenols are usually involved?
How to know the wavelength that i should use to measure the optical rotation? What is the difference between optical rotation and optical ellipticity? thanks
02 March 2016 3,217 7 View
My compound only dissolve in water (glucomoringin). what is the best solvent to dissolve it to run NMR
01 February 2016 7,563 2 View
I'm extracting glucomoringin from plant. when i read in journal, most stated that it must be activated with enzyme before it can be use for any biactivities.The purpose is to hydrolyze the gmg to...
10 November 2015 7,691 4 View
I'd done MTT assay on a cancer cell line to study cytotoxicity of crude plant extracts. The incubation period for the assay were 24, 48 and 72 hours. However, i found that some of the ic 50...
08 September 2015 8,944 4 View
Prostate cancer cell lines in local university. I really need to find those cell lines. thanks
07 August 2015 6,403 3 View
I had purify glucosinolates from seed of plant. But when i do NMR, the result showed impurities in 0-2.5 ppm. How can i solved this issue?
05 June 2015 1,853 5 View
My compound is a glucosinolate. It is easily dissolved in water, but I found out that it isnt dissolved well in organic solvent that i tried such as methanol, ethanol, acetone and hexane I need...
03 April 2015 5,139 6 View
i had done tlc for my crude extract as it has been suggested by sv to check the compound and solvent system. i had found only 1 spot for my crude and the best solvent system. my desired pure...
02 March 2015 9,103 17 View
Why do people spending a lot of money for buying orchid? What are the characteristics of beautiful or characteristics of marketable orchids?
02 March 2014 5,654 1 View
We tried to culture pineapple n36 for some months, but still have fungus contamination. Any suggestion for sterilization techniques?
06 July 2013 8,455 5 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View