I found in literature that authors plot x-axis as emission wavelength and sometimes as excitation wavelength. Is it dependent on spectrophotometer used?
They refer to two different type of experiments. In a typical fluorescence (emission) measurement, the excitation wavelength is fixed and the detection wavelength varies, while in a fluorescence excitation measurement the detection wavelength is fixed and the excitation wavelength is varied across a region of interest.
Hi Domenico,
I am talking about synchronous fluorescence measurement where both the excitation and emission monochromator are scanned simultaneously with a certain wavelength offset.
It doesn't really matter if the difference between the monochromators for the synchronous scan is 30 nm then one can simply add/subtract the 30 nm if they want to change the axis from excitation to emission or vice versa.
If you are unsure how your system works then plot the output from the reference photodiode R1 as well as the signal S1. You know the peak of the xenon lamp is 467 nm. If the plot says its 497 nm then you know your system has plotted x as the emission axis. If the plot says its 467 then you know your system has plotted x as the emission axis...
- Badges
- Science topic
- Similar topics
- Physical Sciences
- Physical Phenomena
- Luminescence
- Fluorescence