Terbium-[Ethyl-4-hydroxy-1-(4-methoxyphenyl)-2-quinolinone-3-carboxylate] complex has been investigated as an analytical probe for pesticide detection, while scanning pesticides, Malathion pesticide show strong quenching to Terbium emission, but in UV spectra, Malathion found to have absorption at the excitation wavelength I had used to get fluorescence. I made stern-vlomer studies which shown that the quenching is static, also I made binding studies and thermodynamic parameters.
My question here? Is my quencher is really has static quenching mechanism or it only inner-filter effect?
If it is a filter effect the magnitude is related to both the concentration and the median distance excitation/emission traves to exit the solution. These effects are testable empirically using fluorescence cells of different dimensions.
Prof. Paul Thank you a lot for your kind response.
Thank you a lot for your kind response.
I have already made complete study of concentration effect of the quencher, get the LOD and LOQ, made Stern-Volmer studies, and binding studies calculate all thermodynamic parameters. then when I send for publication in Journal of Luminescence, the reviewer asked for UV for the probe, the pesticide (quencher) and Probe+pesticide, and he said if the quencher has absorption at the excitation of the probe the then it is simply an inner filter effect (not dynamic or static quenching)! In such a case all Stern-Volmer studies or study of binding parameters will be irrelevant!!
Is this true? as you can notice I attached the UV in my question.
I know you’re very busy so I appreciate any time you can give me.
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