T. equi, primers targeting the 18S rRNA had the sequence 5'-TCGAAGACGATCAGATACCGTCG-3' and the reverse primer Equi-R had the sequence 5'-TGCCTTAAACTTCCTTGCGAT-3', yielding a 392 bp product may be it is useful.best regard

i can design one for you if you like :)

The primers suggested by Dr. Nabeel above are fine.

However, they will provide these yields:

Theileria equi : 435 bp

Theileria luwenshuni : 444 bp

Theileria orientalis : 444 bp

Theileria velifera : 444 bp

Babesia sp. : 442 bp

Sarcocystis sp. : 432-442 bp

Toxoplasma gondii : 432 bp

Zymoseptoria tritici : 1986 bp

So, you could use them for the already isolated Th. equi but not for clinical samples due to close average bands of PCR products among the different parasites.

Furthermore, you need to use 2% gel and a ladder that could differentiate easily the 100-600 bp.

Regards,