A method of simultaneous isolation of nuclear DNA (nDNA) and mitochondrial DNA (mtDNA) from human blood has been proposed by improvising Lahiri's method of isolation of nuclear DNA.

Dear Ravi,

we have been using human whole blood genomic DNA for next generation sequencing and see the mt genome in the mix a few thousand times more often then the nuclear genome. So if your question is about the 37 mt genome encoded genes ant potential mutations by NGS, then a purification may even not be needed. 

Frank

Hi taha ,

Easiest way Qiagen Kit can be used to obtain .

https://www.qiagen.com/in/products/catalog/sample-technologies/protein-sample-technologies/fractionation-and-depletion-kits/qproteome-mitochondria-isolation-kit/

https://www.qiagen.com/in/products/catalog/sample-technologies/protein-sample-technologies/fractionation-and-depletion-kits/qproteome-mitochondria-isolation-kit/

https://www.qiagen.com/in/products/catalog/sample-technologies/dna-sample-technologies/genomic-dna/repli-g-mitochondrial-dna-kit/

Regards

Aamir javed