I used four different substrates and inoculated them with four different types of nano particles. I found that the same microorganisms behave differently in the presence of the same NPs, but with different substrates. What technique/method or explanation can I use for finding the reason of such behavior?
you can prepare an activity test and yu can measure activiy of this bacteria. For example consumption speed of COD can give you an indirect result
Actually, I detect 2 different questions under your generic question.
First Q&A: Which technique/method to find different behaviour? The simplest and best way to find the different behaviour in in different substrates is to assess the methabolic activity of the various bacterias. This can be done through a qualitative and quatitative analysis of the biogas (by gas chromatography, FID detector).
Second Q&A: What explanation to find the reason of behaviour? Evidently the reason is that bacterias use different or specifical enzymes in different substrates so as be able to digest the various organic and inorganic components. The result is that the methabolic products are not the same for different substrates, despite the same bacteria and NPs.
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