How to dissolve cross-linked Amberlite IRC120 H, hydrogen form resin?

Hello all, I have used Amberlite IRC120 H, hydrogen form acidic cation-exchange resin for the ion-exchange of waterglass [sodium silicate solution -- 40% Sodium silicate (mol ratio > 3.2)...

21 February 2021 5,614 3 View

What molecules bind to dsDNA?

Hello, there are some molecules that bind to double-stranded DNA; for instance, SYBR green and some antibodies. Are there more examples? Thank you

25 January 2021 7,595 4 View

What are best conditions for in vitro hybridization?

Hello, I would like to hybridize a probe and an anti-probe (ssDNA oligonucleotides) in a reaction vessel. What would be the best buffer to use? and the temperature of annealing? Thank you

06 December 2020 4,121 0 View

Which is the best statistical approach to an experimental measurement?

Hi everyone, my question is the following. I have to perform an experiment with the aim of finding a correlation between a quantity X (e. g. temperature) and a quantity Y (depending on X). Which...

06 October 2020 3,911 6 View

How to activate polystyrene with carboxyl group?

Is there a simple (that is one that requires few and not very exotic reagents) chemical reaction to "activate" or "functionalize" the polystyrene of a simple microtitre plate? In other words, I...

06 September 2020 233 3 View

What is the stability of EDAC in aqueous solution?

06 September 2020 4,305 3 View

What test to compare shannon index results?

Hello, I have obtained the following Shannon indices from a trio of samples: 27.73 (healthy tissues), 24.55 (primary colon tumor), and 1.20 (metastatic tissues). The indices have been obtained...

30 August 2020 5,543 1 View

What is the concentration of acid for an ELISA stop solution?

Hello, what is the correct molarity of a stop solution for a peroxidase using o-Phenylenediamine dihydrochloride (OPD)? I am getting all sorts of molarities, from fractional up to 3 M according to...

01 July 2020 3,729 1 View

Is there a database for bacterial features?

Hello, is there a repository of the basic characteristics of bacteria, such as maximum growth rate? In the works related to the modelling of bacterial growth, the parameters requested are always...

25 February 2020 5,498 2 View

Can the PCR fail to amplify high loads of target?

Hello, I did the PCR (long-range, target ~5 Kb) that is shown in the attached file. The lanes are: 1. ladder 2. target (plasmid purified from E. coli after transfection), 50 ng/uL corresponding to...

18 February 2020 6,863 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How many of absolute errors (in kcal) do you believe affect the prescription of the diet when estimating the energy required for an elite athlete?

Dear colleagues, I am writing an article that compares the measured and predicted resting metabolic rate in elite athletes. However, to define a cutoff point to determine which equation is more...

16 February 2021 4,515 3 View

Peptide precipitation as pre-step for acid hydrolysis for amino acid analysis?

I'm currently experiencing problems during acid hydrolysis for total amino acid analysis as my first samples contain high amounts of sugar (I get too low results back). I therefore had the idea to...

14 February 2021 6,049 3 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Extraction of phytochemicals from juice?

I am trying to extract phytochemicals from cane juice. I have tried lyophilization and SALLE techniques but the final product is too sticky and sugars are hindering any further extraction. I have...

03 February 2021 8,784 3 View

Alternatives to .45 um filter to isolate MS2 from lysed E. coli?

Hello, I am looking for alternatives or cheaper materials to isolate out MS2 from E. coli following lysis via chloroform. Some literature I have reviewed uses a .45 um nanopore filter following...

02 February 2021 807 3 View

What are some good protocols for native outer membrane protein extraction from E. coli?

I am trying to extract any and all outer membrane proteins from a lab strain of e. coli (ATCC 11229) and am looking for the best general protocol to extract outer membrane proteins. All...

25 January 2021 5,736 3 View

What could be exact way to remove invasive Prosopis juliflora from arid and semi-arid tropics of world where species is considered disaster ?

Prosopis juliflora is considered a noxious weed but its eradication is very costly inhivitive if no impossible ! We have very strong openion that it's spread could be chacked only if it's pods are...

25 January 2021 4,433 7 View