Any good ideas to study and grow the unculturble bacteria?

08 September 2016 385 3 View

Why there are often false positive bacterial colonies on antibiotic plates after DNA transformation?

During DNA transformation experiments, we sometimes see a few false positive transformants ---- bacterial colonies that carry no any insert plasmids or DNA fragments but can still grow on...

07 August 2016 3,664 5 View

Suggestion to increase the transformation efficiency for non-model bacteria?

04 May 2016 2,142 2 View

What is the limiting factor in CO2 reduction by electricity?

There are some reports on reducing CO2 into fuel chemicals but the efficiency is quite low. What do you think is the limiting factor of CO2 reduction? We have to identify the rate-limiting factor...

01 February 2016 1,086 6 View

How can I express and purify oxygen-sensitive enzymes?

08 September 2015 4,760 5 View

How do you screen thermo-stable enzymes from mutant library in a high throughput way?

06 July 2015 9,838 7 View

How do thermophilic bacteria deal with the instability of many coenzymes like NADH and NADPH during their metabolism?

04 May 2015 2,840 4 View

What do you think the potential of cell-free systems in biotechnology?

03 April 2015 244 6 View

Do you know any good academic conferences for bioenergy, biofuels and biotechnology?

03 April 2015 4,683 0 View

Is an enzyme complex encoded by a single gene, an operon or several independent genes?

02 March 2015 9,769 8 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Is there a classification scheme for rock cavities or caverns with respect to their size and shape?

The range of forms and sizes of cavities in rock is very big. Is there a classification scheme for rock cavities regarding their size and shape?

02 March 2021 4,511 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View