What stiffness of polyacrylamide based gel can one use to mimic the human neonatal heart matrix stiffness when culturing hESc derived cardiomyocytes?

Ravindra Kailasrao Zirmire @Ravindra-Zirmire

04 April 2015 1 8K Report

We are culturing hESc derived cardiomyocytes on different substrate of different stiffness to study the effect of matrix stiffness on cytoskeleton organization. I would like to know what stiffness of polyacrylamide based substrate, I can use to mimic the stiffness of normal physiological human neonatal heart.

Ravindra Kailasrao Zirmire

Thanks a lot Yohan for your suggestions.

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