I've used a drug that I am suspecting increases cell migration. I've got data of area at 0 hour and 24h of a scratch assay. I've got many replicates but can't seem to figure out the best strategy for statistical analysis. Any suggestion with some brief explanation?
You can use a t-test if you have only two conditions (control and treated). Here, you have 0hr which can be considered as control and 24hr could be your treated.
PFA.
Article An image J plugin for the high throughput image analysis of ...
https://www.blossombio.com/pdf/products/APP_AN30_Wound_Healing_Data_Analysis.pdf
Regards
Saurabh
Dear Hammad
First, record the number of migrated cells in every replicate for treated and untreated samples using image analysis tools (e.g. ImageJ and the like), Then you can utilize unpaired t-test to make a statistical comparison. However, if you have employed more than one concentration of the drug of interest, you must use one-way ANOVA (followed by tukey's post-test) to compare the performance of different concentrations (including zero concentration=untreated group). I suggest to use GraphPad Prism as a simple and straightforward statistical software.
I'll just mention for other researchers how I approached this problem.
I simply calculated % wound closure for all my sample groups. I had three columns for my three conditions/treatments. Each of these columns had % wound closure replicates. I used a one way ANOVA in graphpad to compare each of my treatments with my negative control to see if it's significant at p
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