How do you deal with large ANSYS Workbench analyses?

Hello, I am trying to evaluate stresses on a model which is quite large and I have memory limitations. I've seen cases ( http://www.padtinc.com/blog/submodeling_ansys_mechanical/ ) where people...

10 July 2020 5,913 3 View

Lamp (bulb) for iCycler iA iQ5 bio-rad..?

Hello everyone, Has anyone ever change the bulb (lamp) in a iCycler iA iQ5 bio-rad? Bio-rad does not sell this lamp anymore, and I'm searching for an alternative solution. However a bulb with...

27 November 2019 2,467 1 View

I was wondering if there are qualitative articles about what leads to impulse buying?

07 October 2019 850 5 View

I'm looking for a PK software that can simulate time-concentration graphs via inputting various variables (ie. Vd, kel, F, etc.)?

Thanks in advance! Purpose is for teaching.

19 August 2019 6,293 1 View

Can any one help me with an analysis grid of school books including indicators on the development of logical reasoning ?

Can any one help me with an analysis grid of school books including indicators on the development of logical reasoning in mathematics, experimental sciences ?

11 June 2019 3,525 4 View

How to blast a fast file with multiple sequences in a genome?

Hello to all, I have a complete genome of a plant and a fast file with multiple sequences of a specific protein ( nucleotide sequence). I want to blast the file into the genome to see if these...

08 February 2019 3,473 5 View

Formvar-coated grids - grey colour, best method?

I need to prepare formvar-coated grids for TEM. I usually use an uncommon method of a drop of formvar on water, however the formvar film is usually uneven. Another and more common method is by...

03 December 2018 2,250 5 View

Long term storage of Cholera Toxin B ?

Hi, I am planning to use Cholera Toxin B for neuroanatomical tracing. The CTB conjugated with Alexa Fluor dyes can be obtained in the solid (500 ug or 100 ug powder) form from suppliers and,...

05 November 2017 8,721 1 View

Hippurate hydrolysis test for Campylobacter jejuni negative after long term storage at -70?

Hippurate hydrolysis test was positive for Campylobacter jejuni ATCC 33560 and for other C. jejuni isolated from stool and other human samples. After 6 month storage at -70 degree hippurrate...

16 May 2017 4,535 4 View

Suggestions for alternative housekeeping genes for western blot ?

We have a knockdown THP-1 cell line that has cytoskeleton and structural proteins affected. We are doing western blots and tried using Actin, Lamin-B and GAPDH as a control but their expression...

09 May 2017 6,811 6 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

How can I make stacked bar graph with scatter plot?

I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?

01 March 2021 9,383 1 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View