I'm going to do IHC in pig brain to detect biomarkers related learning and memory. It's very hard to find biomarkers for pig model. I would like to have some suggestions from fellow researchers who have experience in IHC staining in pig brain.
I've selected an interpretivist philosophy along with a qualitative inductive approach for my research. I plan to employ semi-structured interviews as my primary data collection method. However, I...
12 May 2024 7,816 4 View
Certainly! Here's a refined version of your question: "I've been encountering challenges downloading and reading journal articles. Despite some being accessible through Sci-Hub using their DOI...
12 April 2024 8,975 5 View
I want to extract RNA from boar sperm I used 200mL of semen sample to extract RNA I tried PureLink_RNA_minikit to extract RNA I got the RNA concentration around 5ng/uL I hope to use TOYOBO...
19 April 2022 6,297 3 View
I am going to extract RNA from boar semen samples. I get low concentration of RNA and I wanted to know minimum RNA concentration required to prepare cDNA and do RT-PCR
06 April 2022 7,031 3 View
can we apply ARDL method ,after adding moderated variable into the model, because my sample size is 30 ,and variables are mixed of I(0) and I(0).
23 January 2022 9,508 3 View
I am using the SOD kit from CELL BIOLABS. standard curve I got was totally different from the standard curve illustrated in the protocol. I wanted to know possible reasons and how to overcome this...
11 May 2021 8,362 8 View
I want to detect protein concentration on testis sample. I did Bradford assay using BSA as a standard. I got the standard curve nicely, but my samples are not within the standard curve. Sample...
22 April 2021 3,911 3 View
I wanted to do above assays on mouse spleen samples. So I need to know whether I have to determine the protein concentrations before starting the assays? If I have to, I hope to do BRADFORD assay.
28 March 2021 7,871 6 View
Hello, I want to know the best method that we can use to differentiate THP-1 cells into immature dendritic cells please is anyone there who can help with this?
01 January 1970 5,155 0 View
Dear colleges, i hope to use the ARDL model to examine the impact of FDI on Innovation.All variables are in ratio except the dependent variable. so can I employ these data to run the ARDL model as...
01 January 1970 10,037 1 View
Pig slurry is rich in major and minor nutrients. Is there any way to improve / Enrich its manure quality to be used in agriculture organically ? please share your knowledge.
09 August 2024 5,605 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
Do You Know Untreated TMJ Affects Your Brain And Entire Body?
04 August 2024 6,282 1 View
What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?
01 August 2024 1,722 1 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
Information in The Three Dimensions of Time.Information in The Three Dimensions of Time Information Transfer based on Brains Entanglement.Information Transfer based on Brains Entanglement Time...
28 July 2024 7,522 0 View
Please, what is the memory consumption of the Matlab function quad tree decomposition procedure [S = qtdecomp(I)] with respect to the input set I?
27 July 2024 5,455 2 View
I have found an EEG where only alpha waves are present. Beta waves are not found in active patients. What interpretations ?
26 July 2024 4,741 1 View